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Test Code 022 Semen Analysis

Important Note

Fertility Questionnaire

Medical Associates: Semen Analysis must be scheduled.


Additional Codes


PowerChart: Not orderable

MA Cerner: Semen Analysis.

Specimen Requirement

Semen specimen is required.

Additional Specimen Collection Information

Collect the semen specimen after a 2-7 day period of abstinence. Collect the specimen by masturbation into a sterile container. Care must be taken to collect the entire specimen. Washed containers are not acceptable. In certain situations, the physician may supply the patient with an SCD, seminal collection device, which is a condom designed for seminal fluid collection and evaluation. Ordinary latex condoms must not be used for semen collection because they contain agents that may interfere with the motility of sperm. If there is any question of a specimen's suitability, contact the physician or a pathologist. Protect the sample from extreme temperatures (less than 20°C or greater than 37°C) during transport. For fertility studies the specimen must arrive at the lab within 1 hour of collection and must be examined immediately upon arrival. For fertility studies the specimen must be kept at body temperature (37°C) while being transported to the lab.

Useful For

Determining male fertility status


Testing is performed Monday through Sunday.

Testing must be performed immediately upon receipt of the specimen.


CPT Code Information

89320 Semen analysis; volume, count, motility and differential

LOINC Code Information

54231-6 Semen Analysis Fertility Panel

Test Method


Reference Values

Days Abstinence: 2-7 days
Liquefaction time: Within 60 minutes from time of collection.
Appearance: Normal
Volume: ≥ 1.5 mL
Viscosity: Normal (Nonviscous)
pH: 7 - 8
Agglutination: None
Progressive Motility: ≥ 32% Motile
Sperm Count: ≥ 15 mil/mL
Sperm Morphology: ≥ 4% normal forms
Sperm morphology (in conjunction with sperm concentration and motility) can be used to classify men as subfertile or fertile. None of these measurements, however, are diagnostic of infertility and morphology values in the subfertile range do not exclude the possibility of normal fertility.