Sign in →

Test Code 136 Bilirubin, Fractionated

Additional Codes


PowerChart: Bilirubin Total, Direct and Indirect

MA Cerner: Bilirubin, Fx - Tl/Dir/Ind

Specimen Requirement

0.5 mL plasma or serum is required.

Additional Specimen Collection Information

Collect blood in a lithium heparin, green-top or red-top tube. PST and SST (gel tubes) are acceptable. Serum or plasma should be separated from contact with the cells within 2 hours of collection. Specimens not centrifuged within 4 hours of collection may be rejected. Refrigerate the specimen if unable to assay within 8 hours of collection. Hemolyzed specimens are not acceptable. Samples with >3+ lipemia must be cleared prior to analysis. Protect from light.

Useful For

Assessing liver function

Evaluating a wide range of diseases affecting the production, uptake, storage, metabolism, or excretion of bilirubin

Monitoring the efficacy of neonatal phototherapy



Testing is performed Monday through Sunday.

Routine orders: Typically completed within 4 hours after the specimen arrives at the testing site.

Expedite orders: Typically completed within 1 hour after the specimen arrives at the testing site.

STAT orders: Typically completed within 30 minutes after the specimen arrives at the testing site.

CPT Code Information

82247 Chemistry: Bilirubin; total

82248 Chemistry: Bilirubin; direct

LOINC Code Information

34543-9 Bilirubin Direct And Total Panel [Mass/Volume]-Serum Or Plasma

Test Method


  • Direct: Timed Endpoint, Diazo;Beckman DXC880i
  • Total: Timed Endpoint, Diazo; Beckman DXC880i
  • Indirect: Calculated


  • Direct: Colorimetric Diazo Method; Roche Cobas 6000
  • Total: Colorimetric Diazo Method; Roche Cobas 6000
  • Indirect: Calculated

Reference Values

≥ 5 Days of Age:

  • Total: 0.3-1.2 mg.dL
  • Direct: 0.0-0.2 mg/dL
  • Indirect: 0.0-0.8 mg/dL



Values in premature infants may be higher than in full term infants amd may reach peak concentrations at later times.

Full Term:

  • Total Birth-1 Day: < 5.8 mg.dL
  • Total 1-3 Days: < 8.2 mg/dL
  • Total 3-5 Days: < 11.7 mg/dL
  • Critical Value: Total Bilirubin (Infant-1 Month): ≥ 16.0 mg/dL