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Test Code 419 Vitamin D 25-Hydroxyvitamin D2 and D3

Important Note

Human Anti-Mouse Antibodies (HAMA) may be present in samples from patients who have received immunotherapy or diagnostic procedures utilizing monoclonal antibodies or in individuals who have been regularly exposed to animals. Additionally, other heterophile antibodies, such as human anti-goat antibodies may be present in patient samples. Manufacturers formulate assays to minimize the effects of these antibodies; however careful evaluation of patient results must be done when patients are known to have such antibodies.

Additional Codes


PowerChart: Vitamin D 25-Hydroxy D2 D3 Level

MA Cerner: Vitamin D2/D3, 25-OH

Specimen Requirement

1 mL serum or plasma is required.

Additional Specimen Collection Information

Collect blood in a lithium heparin, green-top or red-top tube. PST and SST (gel tubes) are acceptable. Serum or plasma should be separated from contact with the cells within 2 hours of collection. Refrigerate the specimen if unable to assay within 72 hours of collection. Hemolyzed specimens are not acceptable. Specimens with >1+ lipemia must be cleared prior to analysis.

Useful For

Diagnosis of vitamin D deficiency

Differential diagnosis of causes of rickets and osteomalacia

Monitoring vitamin D replacement therapy

Diagnosis of hypervitaminosis D



Testing is performed Monday through Sunday.

Routine: Typically completed within 4 hours after the specimen arrives at the testing site.

STAT: Typically completed within 1 hour 10 minutes after the specimen arrives at the testing site.

CPT Code Information

82306 Chemistry: Vitamin D; 25 hydroxy, includes fractions, if performed


BCBS requires a signed ABN. (Frequency limit of 1 per calendar year.)

LOINC Code Information

62292-8 25-Hydroxyvitamin D2+25-Hydroxyvitamin D3 [Mass/volume] in Serum or Plasma

Test Method

MercyOne: Two-Step Competitive Binding Immunoenzymatic Assay, Beckman

MA: Immunoenzymatic ("Sandwich") Chemiluminescent Method; Roche Cobas 6000

Reference Values

30-100 ng/mL

  • Deficient: <20 ng/mL
  • Insufficient: 20 - <30 ng/mL
  • Sufficient: 30-100 ng/mL
  • Upper Safety Limit: >100 ng/mL

Vitamin D deficiency is a cause of secondary hyperparathyroidism which results in impaired bone metabolism (e.g. Osteoporosis). Reduced Vitamin D has also been associated with increasing risk of many chronic disorders including cancers, autoimmune and cardiovascular disease. Patients that live in northern climates who have limited sunlight exposure are more likely to have Vitamin D deficiencies. The major storage form of Vitamin D is 25-Hydroxyvitamin D.