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Test Code 473 HIV 1 and 2 Antibody, p24 Antigen

Important Note

This test should not be performed on newborns or infants less than 24 months old due to false positives from circulating maternal antibodies and the lack of antibody production in this age range.

Iowa state law no longer requires documentation of consent for Human Immunodeficiency Virus (HIV) testing.

  • Testing may or may not be performed anonymously.
  • Samples collected previously may be used.
  • Referring stations will be solely liable for the documentation of counseling, when applicable.
  • Referring stations will place orders for hiv testing in accordance with their established protocol. If anonymity is requested a unique identification will be assigned by the referring station. 
  • MercyOne will accept exactly what is submitted. If a proper name does not appear on the sample tube and requisition, MercyOne will assume that the client has requested anonymity.
  • The specimen must be labeled with the subject’s unique ID as assigned by the referring station and a second patient identifier (e.g. birthdate). The label will also contain the date and time of acquisition and the initials of the phlebotomist.
  • HIV-1/HIV-2 antibody is performed. If reactive, the test will be referred to Mayo Medical Laboratories for confirmatory testing (HIV-1 and HIV-2 Antibody Confirmation and Differentiation, Plasma HVDIP) at an extra charge.
Infectious

Additional Codes

ALAB: HIVAB

PowerChart: HIV 1&2 Antibody, HIV-1 p24 Antigen

MA Cerner: HIV1/2 p24 AG

 

Confirmatory Testing: refer to Mayo code HVDIP (HIV-1 and HIV-2 Antibody Confirmation and Differentiation, Plasma)

Test Method

Two-Step Immunoassay Using CMIA Technology with Flexible Assay Protocols Referred to as Chemiflex

Specimen Requirement

2.0 mL EDTA plasma is the specimen of choice for HIV testing. This is the required sample type for confirmation sent to Mayo.

Serum, SST, and heparin plasma are also acceptable specimen types.
NOTE: Serum and heparin plasma are not acceptable specimen types for confirmation sent to Mayo.

Additional Specimen Collection Information

Collect blood in a purple-top EDTA tube. Specimens may be stored at room temperature for up to 3 days, refrigerated for up to 7 days, and frozen for extended storage. Samples with >1+ lipemia must be cleared prior to analysis.

Performance

Testing is performed Monday through Sunday.

Routine: Typically nonreactive tests are completed within 4 hours after the specimen arrives at the testing site. Repeatedly reactive samples require confirmatory testing referred to Mayo Medical Laboratory.

STAT: Typically completed within 1 hour after the specimen arrives at the testing site.

Reference Values

Nonreactive

This fourth generation HIV assay simultaneously functions as both a third generation immunoassay for the detection of IgG and IgM antibodies and for the direct detection of p24 antigen.

CPT Code Information

87389 Enzyme Immunoassay Technique by Organism: HIV-1 antigen(s), with HIV-1 and HIV-2 antibodies, single result

 

G0475: 
This HCPC code has frequency limitations. Only 1 HIV screening is allowed annually. A full 11 months must elapse following the month in which the previous test was performed in order for the subsequent test to be covered. EXCEPTION: 3 HIV screenings for pregnant Medicare beneficiaries are allowed when (1) the diagnosis of pregnancy is known, (2) during the third trimester, and (3) at labor, if ordered by the woman’s clinician.

  1. For pregnancy screens, Z11.4 must be the primary diagnosis with a secondary diagnosis of Z34.00, Z34.01, Z34.02, Z34.03, Z34.90, Z34.81, Z34.82, Z34.83, Z34.90, Z34.91, Z34.92, Z34.93, O09.90, O09.91, O09.92, or O09.93
  2. Z11.4 must be the primary diagnosis code for Medicare beneficiaries between ages 15 and 65.
  3. Z11.4 can be used for patients younger than 15 and older than 65 ONLY WHEN one of the following secondary diagnosis codes is included: Z72.51, Z72.89, Z72.52, or Z72.53

Useful For

Screening for infection with Human Immunodeficiency Virus

LOINC Code Information

56888-1 HIV 1+2 Ab+HIV1 p24 Ag [Presence] In Serum By Immunoassay