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Test Code BAFS Bile Acids, Fractionated and Total, Serum

Additional Codes


Powerchart: Bile Acids Fractionation

MA Cerner: Bile Acids Fractionation

Ordering Guidance

This test is useful in diagnosing intrahepatic cholestasis of pregnancy and does not support the assessment of either peroxisomal biogenesis disorders or inborn errors of bile acid metabolism.


For diagnostic testing for peroxisomal biogenesis disorders, order BAIPD / Bile Acids for Peroxisomal Disorders, Serum.

Specimen Required

Patient Preparation: Patient must be fasting for 12 to 14 hours.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Useful For

Measuring tauro- and glycol-conjugated and unconjugated bile acid constituents in serum


Monitoring patients receiving bile acid therapy, such as cholic acid, deoxycholic acid, or ursodeoxycholic acid


Aiding in the evaluation of liver function; evaluation of liver function changes before the formation of more advanced clinical signs of illness such as icterus


Determining hepatic dysfunction as a result of chemical and environmental injury


Indicating hepatic histological improvement in chronic hepatitis C patients responding to interferon treatment


Indicating intrahepatic cholestasis of pregnancy


This assay is not useful for the diagnosis of peroxisomal biogenesis disorders or inborn errors of bile acid metabolism.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Bile Acids, Fractionated and Tot, S

Specimen Type


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 90 days
  Ambient  90 days
  Frozen  90 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

Total cholic acid: ≤5.00 nmol/mL Total chenodeoxycholic acid: ≤6.00 nmol/mL Total deoxycholic acid: ≤6.00 nmol/mL Total ursodeoxycholic acid: ≤2.00 nmol/mL Total bile acids: ≤19.00 nmol/mL

Day(s) Performed

Monday through Friday

Report Available

2 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
BAFS Bile Acids, Fractionated and Tot, S 43130-4


Result ID Test Result Name Result LOINC Value
35796 Total Cholic acid 30518-5
35797 Total Chenodeoxycholic acid 30519-3
35798 Total Deoxycholic acid 30520-1
35799 Total Ursodeoxycholic acid 55159-8
35800 Total bile acids 14628-2