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Test Code FFN Fetal Fibronectin, Rapid

Important Note

The Hologic Specimen Collection Kit available from the laboratory is the only acceptable collection device for this test.

Additional Codes

Powerchart: Fetal Fibronectin
MA Cerner: Fetal Fibronectin, Rapid

Specimen Requirement

Cervicovaginal Secretions

Additional Specimen Collection Information

  1. Specimen Collection:
    1. Obtain the specimen using the Hologic Specimen Collection Kit. (This is the only acceptable collection device.) The specimen is obtained from the posterior fornix of the vagina during speculum examination. The polyester-tipped applicator provide in the specimen collection kit is inserted into the vagina and lightly rotated across the posterior fornix for approximately 10 seconds to absorb the cervicovaginal secretions.
    2. Once the specimen is obtained, carefully remove the applicator from the vagina and immerse the tip in the tube of buffer provided with the specimen collection kit.
    3. Break the shaft (as the score) even with the top of the tube. Align the shaft with the hole inside the tube cap and push down tightly over the shaft, sealing the tube.
    4. Label the tube with the patient’s name, date of birth and date and time of collection.
  2. Specimen Storage and Transport:
    1. Specimens that are not tested within eight (8) hours of collection must be stored refrigerated at 2-8oC and assayed within three (3) days of collection, or frozen and assayed within three (3) months. Store appropriately and avoid extreme temperatures.
    2. Transport specimens at 2-25oC, or frozen.

Useful For

Detection of fFN in cervicovaginal secretions between 24 and 34 completed weeks of gestation is reported to be associated with preterm delivery in symptomatic and asymptomatic pregnant women.


Testing is performed Monday through Sunday.
Routine: Typically completed within 4 hours after the specimen arrives at the testing site.
STAT: Typically completed within 30 minutes after the specimen arrives at the testing site.

CPT Code Information

82731  Fetal Fibronectin, cervicovaginal secretions, semi-quantitative

LOINC Code Information

20404-0  Fibronectin Fetal Vag Ql

Test Method

Lateral Flow, Solid-Phase Immunochromatographic Assay

Reference Values


Reported as Negative, Positive or Equivocal