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Test Code REPU Electrophoresis, Protein, Random, Urine

Additional Codes


Powerchart: Protein Electrophoresis Random Urine


Ordering Guidance

Random urine specimens may be sufficient for identifying monoclonal proteins, but 24-hour specimens should be used to quantitate and monitor urinary abnormalities. See MPSU / Monoclonal Protein Study, 24 Hour, Urine.

Shipping Instructions

Refrigerate specimen after collection and send refrigerated.

Specimen Required


-Urine Container, 60 mL (T313)

-Sarstedt 5 mL Aliquot Tube (T914)

Submission Container/Tube: Plastic, 60-mL urine bottle and plastic, 5-mL tube

Specimen Volume: 50 mL

Collection Instructions:

1. Collect random urine specimen.

2. Aliquot at least 25-mL specimen in plastic, 60-mL urine bottle and at least 1-mL of specimen in plastic, 5-mL tube.

3. Label specimens appropriately (60-mL bottle for protein electrophoresis and 5-mL tube for protein, total).

Secondary ID


Useful For

Identifying monoclonal gammopathies using random urine specimens

Profile Information

Test ID Reporting Name Available Separately Always Performed
RPEU Protein Electrophoresis, Random, U No Yes
PTCON Protein, Total, Random, U No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RIFXU Immunofixation, Random, U No No

Testing Algorithm

Urine protein electrophoresis alone is not considered an adequate screening for monoclonal gammopathies. If a discrete electrophoresis band is identified, the laboratory will evaluate the urine protein electrophoresis and, if necessary, perform immunofixation at an additional charge.


The following algorithms are available:

-Amyloidosis: Laboratory Approach to Diagnosis

-Multiple Myeloma: Laboratory Screening

Method Name

PTCON: Turbidimetry

RPEU: Agarose Gel Electrophoresis

RIFXU: Immunofixation

Reporting Name

Electrophoresis, Protein, Random, U

Specimen Type


Specimen Minimum Volume

25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  5 days
  Ambient  24 hours

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values


No reference values apply to random urine.



The following fractions, if present, will be reported as mg/dL:






No reference values apply to random urines.

Day(s) Performed

Total Protein: Monday through Sunday

Protein Electrophoresis: Monday through Friday

Report Available

4 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information



86335-Immunofixation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
REPU Electrophoresis, Protein, Random, U 34539-7


Result ID Test Result Name Result LOINC Value
PTCON Protein, Total, Random, U 2888-6
607975 Albumin 6942-7
607976 Alpha-1 globulin 9734-5
607977 Alpha-2 globulin 38190-5
607978 Beta globulin 9744-4
607979 Gamma globulin 9745-1
33044 A/G Ratio 44293-9
33045 M spike 40661-1
33046 M spike 40661-1
33047 Impression 49299-1