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Test Code SEMB Semen Analysis with Strict Morphology, Semen

Additional Codes


PowerChart: Not orderable

MA Cerner: Semen Analysis.

Useful For

Determining male fertility status


Selecting the most cost-effective therapy for treating male-factor infertility


Quantifying the number of germinal and white blood cells per milliliter of semen

Profile Information

Test ID Reporting Name Available Separately Always Performed
FER Semen Analysis No Yes
MSTC Strict Criteria Sperm Morphology Yes Yes

Reporting Name

Semen Analysis with Strict Morphology

Specimen Type


Ordering Guidance

This test should not be used to check patient’s sterility following a vasectomy. For such cases, order POSV / Post Vasectomy Check, Semen.


Semen analysis specimens submitted to Mayo Clinic Laboratories are not acceptable for fructose testing due to the use of dilution media. For specimen requirements for fructose testing in azoospermia patients, see FROS2 / Fructose, Qualitative, Semen.


Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.

Shipping Instructions

Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends.

Necessary Information

Include the following information:

-Semen volume (required)



-Appearance (color)

-Number of days of sexual abstinence

Specimen Required

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.

Supplies: Semen Analysis Kit - Dilution Media (T178)

Specimen Volume: Total ejaculate

Collection Instructions:

1. After collection, allow the specimen to liquefy for 1 hour.

2. Measure the volume.

3. Place the specimen into media within 1 hour.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Semen Ambient 36 hours

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values


Appearance: normal

Volume: ≥1.5 mL

pH: ≥7.2

Motile/mL: ≥6.0 x 10(6)

Sperm/mL: ≥15.0 x 10(6)

Motility: ≥40%

Grade: ≥2.5

Note: Multiple laboratory studies have indicated that semen parameters for motility and grade on average retain 80% of original parameters when our shipping method is used for transport. Using these averages, samples with 32% to 39% motility and grade of 2 may be in the normal range if testing was performed shortly after collection. Therefore, these borderline patients may need to collect another sample at a local fertility center to verify fertility status.

Motile/ejaculate: ≥9.0 x 10(6)

Viscosity: ≥3.0

Agglutination: ≥3.0

Supravital: ≥58% live

Fructose: positive

Note: Fructose testing cannot be performed on semen analysis specimens shipped through Mayo Clinic Laboratories. If patient is azoospermic, refer to FROS2 / Fructose, Qualitative, Semen. Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.



Normal forms: ≥4.0% normal oval sperm heads

Germ cells: <4 x 10(6) (normal)

≥4 x 10(6)/mL (elevated germinal cells in semen are of unknown clinical significance)

White blood cell count:

<1 x 10(6) (normal)

≥1 x 10(6)/mL (elevated white blood cells in semen are of questionable clinical significance)

Day(s) Performed

Monday through Friday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

89310-Semen Analysis

89398-Strict Criteria Sperm Morphology

If both components performed,

89322-Semen Analysis with Strict Morphology

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SEMB Semen Analysis with Strict Morphology 54231-6


Result ID Test Result Name Result LOINC Value
OVAL2 Strict Morph NL 10622-9
ACRSM Acrosom Defect 66494-6
ABSTN Abstinence 10587-4
CLST1 Collection Site 56816-2
HDSAB Head Shape Abnormal 66495-3
HDZAB Head Size Abnormal 66496-1
TY Study Type 54453-6
CNTN Container Type 74384-9
MD Midpiece Defect 10603-9
TAILD Tail Defect 10604-7
DBLF Double Forms 66497-9
MULTI Multiple Defects 66498-7
APP3 Appearance 13359-5
VL53 Semen Volume 3160-9
GERM3 Germ Cells/mL 10576-7
WBC6 WBC/mL 10579-1
PH1 pH 2752-4
CMT56 Comment 48767-8
MOTML Motile/mL 42531-4
SPML Sperm/mL 9780-8
MOTY Motility 6800-7
GR2 Grade 13942-8
MOTEJ Motile/Ejaculate 6800-7
VISC Viscosity 32789-0
AGGLU Agglutination 33217-1
STAIN Supravital Stain 4466-9
FRCT Fructose 13943-6
CMT45 Comment 48767-8

Specimen Minimum Volume

See Specimen Required

Method Name

FER: Manual

MSCT: Kruger Criteria Strict Morphology

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Secondary ID