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Mayo Clinic Laboratories

Test Code JO1 Jo-Antibody

Important Note

Testing for Scl 70 antibodies is not useful in patients without demonstrable antinuclear antibodies.

Additional Codes

ALAB: JO1

Powerchart: JO1 Antibody

MA Cerner: Jo 1 Antibodies, IgG

Test Method

Multiplex Flow Immunoassay; BioRad BioPlex

Specimen Requirement

0.5 mL of serum. Gel tubes are preferred.

Additional Specimen Collection Information

Collection Instructions:

  • Minimum volume: 500 mcL
  • Hemolysis: Samples with hemolysis >2+ are not acceptable
  • Lipemia: Samples with lipemia >3+ must be ultracentrifuged prior to analysis
  • Icterus: No interference
  • Storage: Stable for up to 7 days at 2-8°C.

Performance

Testing is performed Monday through Friday.

Reference Value

Jo-1 Antibody: Negative (<1 AI) Reported as an antibody index.

CPT Code Information

86235-Jo-1 Antibody

Useful For

Evaluating patients with signs and symptoms of scleroderma and other connective tissue diseases in whom the test for antinuclear antibodies is positive.
A positive test result for Scl 70 antibodies is consistent with a diagnosis of scleroderma.

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